#Camera not recognized in mixscope skin#

If the digital camera white balance function is not activated and applied to the current microscope configuration, images captured under these conditions also appear to have an overall yellow cast ( Figure 1(a)). Without a color conversion filter, the DIC specimen in Figure 1 appears neutral gray, but exhibits an obvious global yellow hue, characteristic of incandescent illumination, when observed through the microscope eyepieces. In the eyepieces, the cell culture appears neutral gray in color when the Nomarski prism is adjusted to achieve the optical path difference illustrated in Figure 1, and a color conversion filter is added to the light path to raise the color temperature of the tungsten-halogen lamp from 3200K to approximately 5500K (daylight value).

#Camera not recognized in mixscope skin#

The specimen is a monolayer culture of adherent Indian Muntjac deer skin fibroblast cells viewed in differential interference contrast ( DIC) at relatively low (one-twentieth of a wavelength) bias retardation. Presented in Figure 1 are a series of digital images recorded of the same microscope viewfield under varying conditions of illumination color temperature. One factor contributing to this discrepancy is that, during imaging, a considerable accommodation of the human visual system to variations in imaging conditions occurs subconsciously, and color rendition problems are usually not realized until an image is recorded and the static version evaluated, often in a different viewing environment. When a captured digital image is critically compared to the image observed through the microscope eyepieces or viewed live on a computer monitor, color variations are often quite striking, and attempts to reconcile differences between the two can be frustrating.